Dr. Chunxian Chen
Contact Information
Citrus Research and Education Center
Lake Alfred, Florida
Citrus Genomics
Phone: 863.956.1151 ext. 1407
Fax (863) 956-4631
Email: cxchen@ufl.edu
Research
Genomics and bioinformatics: Genome linkage mapping; Comparative mapping; Sequence assembly, annotation and motif mining; Genome alignment and comparison; EST SSR and SNP mining and development pipelines; Development of parsing and mining program in Java; etc.
Gene cloning and expression profiling: DNA marker development and genotyping technology; Gene and QTL mapping; Map-based cloning of resistance genes; Homologous gene cloning; Library construction and screening; Physical Mapping; Microarray gene expression profiling; etc.
Breeding and selection in citrus: Identification of origin and ploidy level of sexual and somatic progeny; Genetic characterization and selection of 2n egg derived seedless triploids; Development of binary vectors and constructs for transformation; Genetic improvement of disease resistance; Scalable marker-assisted selection; etc.
Refereed journal articles
Chen C, Grosser JW, Calovic M, Serrano P, Pasquali G, Gmitter J, Stover E, Salvatore J, Gmitter Jr. FG (2008) Verification of mandarin + pummelo somatic hybrids by EST-SSR marker analysis. J. Amer. Soc. Hort. Sci. 133: 794-800.
Chen C, Bowman KD, Choi YA, Dang PM, Rao MN, Huang S, Soneji JR, McCollum TG, Gmitter Jr. FG (2008) EST-SSR genetic maps for Citrus sinensis and Poncirus trifoliata. Tree Genetics and Genomes 4: 1-10.
Chen C, Lyon MT, O'Malley D, Federici CT, Gmitter J, Grosser JW, Chaparro JX, Roose ML, Gmitter Jr. FG (2008) Origin and frequency of 2n gametes in Citrus sinensis x Poncirus trifoliata and their reciprocal crosses. Plant Science 174: 1-8.
Rao M, Soneji J, Chen C, Huang S, Gmitter Jr. FG. (2008) Characterization of zygotic and nucellar seedlings from sour orange-like citrus rootstock candidates using RAPD and EST-SSR markers. Tree Genetics and Genomes 4: 113-124.
Chen C, Zheng QF, Xiang X, Soneji JR, Huang S, Choi YA, Rao MN, Gmitter Jr. FG (2007) Development of pGreen-derived GFP binary vectors for citrus transformation. HortScience 42: 7-10
Chen C, Zhou P, Choi YA, Huang S, Gmitter Jr. FG (2006) Mining and characterizing microsatellites from citrus ESTs. Theoretical and Applied Genetics 112: 1248-1257.
Xiang X, Zheng Q, Huang S, Chen C, Gmitter Jr. FG, Deng Z (2005) Development of RLK-derived molecular markers associating with the resistance to citrus canker [Xanthomonas axonopodis pv. Citri (Xac)]. Molecular Plant Breeding 3: 821-828.
Yu C, Huang S, Chen C, Deng Z, Ling P, Gmitter Jr. FG (2002) Factors affecting Agrobacterium-mediated transformation and regeneration of sweet orange and citrange. Plant Cell Tissue and Organ Culture. 71:147-155.
Deng Z, Tao QZ, Chang YL, Huang S, Ling P, Yu CH, Chen C, Gmitter Jr. FG, Zhang HB (2001) Construction of a bacterial artificial chromosome (BAC) library for citrus and identification of BAC contigs containing resistance gene candidates. Theoretical and Applied Genetics 102(8): 1177-1184
Deng Z, Huang S, Ling P, Chen C, Yu CH, Weber CA, Moore GA, Gmitter Jr. FG (2000) Cloning and characterization of six families of resistance gene candidate sequences in citrus. Theoretical and Applied Genetics 101(5-6): 814-822
Chen C, Yan HH, Zhai WX, Zhu LH, Sun JS (2000) Identification and chromosomal location of a new tandemly repeated DNA in maize. Genome 43: 181-184
Chen C, Gmitter Jr. FG (1999) Direct cloning and sequencing of bacterial artificial chromosome (BAC) insert ends based on double digestion. Plant Molecular Biology Reporter 17: 231-238
Non-refereed papers
Chen C, Zheng QF, Soneji JR, Xiang X, Choi YA, Huang S, Rao MN, Gmitter Jr. FG (2007) Development of GFP vectors for citrus transformation. Acta Horticulturae 738:207-211
Choi YA, Chen C, Huang S, Rao MN, Soneji JR, Gmitter Jr. FG (2007) Identification of AFLP markers associated with citrus canker resistance within Citrus. Acta Horticulturae 738: 201-206.
Conference presentations
Grosser JW, Chen C, Gmitter J, Gmitter Jr. FG (2007) Microsatellite genotyping of seedlings from somatic hybrid and ‘Tetrazyg’ citrus rootstock candidates to determine maternal or zygotic origin. ASHS Annual Conference, July 16-19, Scottsdale, Arizona
Chen C, Choi YA, Soneji JR, Huang S, Rao MN, Wendell MK, Gmitter Jr. FG (2007) Optimization of multiplex PCR using microsatellite primers with a universal adaptor. Plant & Animal Genome XV, San Diego, CA, January 13-17, 2007
Lyon MT, Federici CT, Kacar Y, Chen C, O'Malley D, Chaparro JX, Gmitter Jr. FG, Roose ML (2007) SSR-based linkage maps for sweet orange and trifoliate orange. Plant & Animal Genome XV, San Diego, CA, January 13-17, 2007
Luro F, Costantino G, Billot C, Froelicher Y, Morillon R, Ollitrault P, Terol J, Talon M, Gmitter Jr. FG, Chen C (2007) Genetic maps of Clementine mandarin and intergeneric hybrid Clementine X Poncirus using genomic and EST microsatellite markers. Plant & Animal Genome XV, San Diego, CA, January 13-17, 2007
Chen C, Choi YA, Burrage AD, Soneji JR, Rao MN, Huang S, Gmitter Jr. FG (2006) Aligning citrus unigenes with Arabidopsis and rice chromosome sequences. Plant & Animal Genome XIV, San Diego, CA, January 14-18, 2006
Xiang X, Deng Z, Zheng Q, Huang S, Chen C, Gmitter Jr. FG (2005) Development of RGC markers associated with the citrus canker resistance. Plant & Animal Genome XII, San Diego, CA, January 10-14, 2004
Chen C, Deng Z, Ling P, Yu CH, Huang S, Wendell MK, Gmitter Jr. FG (2000) Screening of overlapping citrus BAC clones with SCAR and RAPD primers. International Society of Citriculture Congress 2000, Orlando, FL, December 3-7, 2000
Chen C, Huang S, Ling P, Yu CH, Deng Z, Gmitter Jr. FG (2000) Characterization of a citrus BAC DNA sequence harboring a NBS-LRR gene cluster and copia-like retrotransposon. Plant & Animal Genome VIII, San Diego, CA, January 9-12, 2000
Chen C, Deng Z, Huang S, Ling P, Yu CH, Gmitter Jr. FG (1999) Screening of a citrus BAC library by PCR for physical mapping of the CTV resistance gene region. Plant & Animal Genome VII, San Diego, CA, January 17-21, 1999
Book chapters
Gmitter Jr. FG, Chen C, Rao MN, Soneji JR (2007) Citrus fruits, pp 265-279, In: Fruits and Nuts, Genome Mapping & Molecular Breeding in Plants, Volume (4), Chitta R Kole, (ed), Springer, Heidelberg
Gmitter Jr. F.G., Deng Z, Chen C (2006) Cloning and characterization of disease resistance genes in Citrus. In: Citrus Genetics, Breeding and Biotechnology. I. H. Kahn (Ed.). CAB International.
