Dr. Manjul Dutt
Research Assistant Scientist
Our research is primarily focused on citrus improvement.
Citrus cell cultures and seedling derived epicotyl explants are being utilized for the genetic improvement of commercially important citrus cultivars. Novel engineered citrus lines are being produced for:
1. Disease management (primarily Huanglongbing and Citrus Canker)
2. Insect management (Asian Citrus Psyllid, Citrus Burrowing Nematode and Diaprepes)
3. Regulation of Flowering (Juvenility reduction)
4. Biofortification (Increase in fruit anthocyanin content)
Conventional breeding methods as well as somatic cell fusion techniques are been used to produce a sizeable population of Citrus x Microcitrus hybrids (2x, 3x and 4x). Trees are being evaluated for tolerance to Huanglongbing. We are also evaluating the rootstock and nutritional requirements for Microcitrus cultivation (primarily Finger Limes) under the central Florida conditions. Results from this research should help Florida growers assess the Finger Lime as a viable addition to their citrus portfolio.
Ph.D. Horticulture, University of Florida.
Dissertation Title: Methods to genetically transform grapevines
M.S. Plant and Soil Science, University of Kentucky.
B.Sc. Agriculture, Orissa University of Agriculture and Technology, Bhubaneswar, India.
Dutt, M., L. Erpen, G. Ananthakrishnan, R.H. Brlansky, I. Maiti and J.W. Grosser. 2016. Comparative Expression Analysis of Five Caulimovirus Promoters in Citrus. Plant Cell Organ Tissue Culture 126:229–238.
Dutt, M., D. Stanton and J.W. Grosser. 2016. Ornacitrus: Development of Genetically Modified Anthocyanin Expressing Citrus with both Ornamental and Fresh Fruit Potential. Journal of the American Society for Horticultural Sciences 141:54-61.
Orbović, V., M. Ćalović, M. Dutt, J.W. Grosser and G. Barthe. 2015. Production and characterization of transgenic Citrus plants carrying p35 anti-apoptotic gene. Scientia Horticulturae 197:203-211.
Dutt M., G. Barthe, M. Irey, J. Grosser. 2015. Transgenic Citrus Expressing an Arabidopsis NPR1 Gene Exhibit Enhanced Resistance against Huanglongbing (HLB; Citrus Greening). PLoS ONE 10(9): e0137134. doi: 10.1371/journal.pone.0137134.
Dutt, M., S.A. Dhekney, L. Soriano, R. Kandel, and J.W. Grosser. 2014. Temporal and spatial control of gene expression in horticultural crops. Horticulture Research 1:14047
Bergey, D., R. Kandel, B. K. Tyree, M. Dutt and S. A. Dhekney. 2014. Role of Calmodulin and Related Proteins in Plant Cell Function: An Ever-Thickening Plot. Springer Science Reviews 2:145–159.
Grosser, J.W., D. Kainth and M. Dutt. 2014. Production of Colchicine Induced Autotetraploids in Pummelo (Citrus grandis) via Indirect Organogenesis. Hortscience 49 (7): 944-948.
Orbovic, V., M. Dutt, and J. W. Grosser. 2013. Evaluation of the germination potential of citrus seeds during the harvesting season. HortScience 48 (9): 1197-1199.
Shen, X., V. Orbovic, M. Dutt, W. S. Castle and F. G. Gmitter, Jr. 2013. Direct shoot organogenesis in Murraya paniculata (L.) Jack – a prerequisite for genetic transformation. HortScience 48(7): 938-941
Dutt M., G. Ananthakrishnan, M.K. Jaromin, R.H. Brlansky and J.W. Grosser. 2012. Evaluation of four phloem-specific promoters in vegetative tissues of transgenic citrus plants. Tree Physiology 32(1):83-93. PDF
Mondal, S. N., M. Dutt, J.W. Grosser and M. M. Dewdney. 2012. Transgenic citrus expressing the antimicrobial gene attacin E (attE) reduces the susceptibility of ‘Duncan’ grapefruit to the citrus scab caused by Elsinoë fawcettii. European Journal of Plant Pathology 133: 391-404. PDF
Chae. C.W., M. Dutt, S. H. Yun, J. H. Park and D. H. Lee. 2011. Development of a SCAR marker linked to male fertility traits in ‘Jinkyool’(Citrus sunki). Journal of Life Science 21(12): 1659-1665. PDF
Dutt M., M. Vasconcellos and J.W. Grosser. 2011. Effects of antioxidants on Agrobacterium-mediated transformation and accelerated production of transgenic plants of Mexican lime (Citrus aurantifolia Swingle). Plant Cell, Tissue and Organ Culture 107:79-89. PDF
Orbovic, V., M. Dutt, and J. W. Grosser. 2011. Seasonal effects of fruit age on regeneration potential and transformation success rate in three citrus cultivars. Scientia Horticulturae 127: 262-266. PDF
Dutt, M., D. H. Lee and J. W. Grosser. 2010. Bifunctional selection-reporter systems for genetic transformation of citrus: mannose and kanamycin based systems. In Vitro Cellular and Developmental Biology – Plant 46: 467-476. PDF
Dutt, M. and J.W. Grosser. 2010. An embryogenic suspension cell culture system for Agrobacterium-mediated transformation of citrus. Plant Cell Reports 29:1251-1260. PDF
Dutt M., M. Vasconcellos, K. Song, F. Gmitter, J.W.Grosser. 2010. In vitro production of autotetraploid Ponkan mandarin (Citrus reticulata Blanco) using cell suspension cultures. Euphytica 173:235-242. PDF
Dutt, M., J. Madhavaraj and J. W. Grosser. 2010. Agrobacterium tumefaciens-mediated genetic transformation and plant regeneration from a complex tetraploid hybrid citrus rootstock. Scientia Horticulturae 123:454-458. PDF
Biswas, M. K., M. Dutt, U. K. Roy, R. Islam and M. Hossain. 2009. Development and evaluation of in vitro somaclonal variation in strawberry for improved horticultural traits. Scientia Horticulturae 122:409-416. PDF
Dutt, M. and J.W. Grosser.2009. Evaluation of parameters affecting Agrobacterium-mediated transformation of Citrus. Plant Cell, Tissue and Organ Culture 98: 331-340. PDF
Dutt, M., Z.T. Li, S.A. Dhekney and D.J. Gray. 2008. A co-transformation system to produce transgenic grapevines free of marker genes. Plant Science 175 (3):423-430. PDF
Geneve, R.L. and M. Dutt. 2008. Using sequential digital images to study seed germination and dormancy. Propagation of Ornamental Plants 8(1):13-16. PDF
Dhekney, S.A., Z.T. Li, M. Dutt and D.J. Gray. 2008. Agrobacterium-mediated transformation of embryogenic cultures and plant regeneration in Vitis rotundifolia Michx. (Muscadine grape). Plant Cell Reports 27(5):865-72. PDF
Li, Z.T., S.A. Dhekney, M. Dutt and D.J. Gray. 2008. An improved protocol for Agrobacterium-mediated transformation of grapevine. Plant Cell, Tissue and Organ Culture 93(3):311-321. PDF
Dutt M., Z.T. Li, S.A. Dhekney and D.J. Gray. 2007. Transgenic plants from shoot apical meristems of Vitis vinifera L. “Thompson Seedless” via Agrobacterium-mediated transformation. Plant Cell Reports 26(12):2101-2110. PDF
Dutt, M. and R. L. Geneve. 2007. Time to radicle protrusion does not correlate with early seedling growth in individual seeds of impatiens and petunia. Journal of the American Society for Horticultural Sciences 132: 423-428. PDF
Li, Z. T., S. A. Dhekney, M. Dutt, M. Van Aman, J. Tattersall, K. T. Kelley, and D. J. Gray. 2006. Optimizing Agrobacterium-mediated transformation of grapevine. In Vitro Cellular and Developmental Biology – Plant 42(3): 220–227. PDF
Downie, A. B., L. M. A. Dirk, Q. Xu, J. Drake, M. Dutt, A. Levy, D. A. Butterfield, R. L. Geneve, J. W. Corum III, K. G. Lindstrom, and J. C. Snyder. 2004. A physical, enzymatic, and genetic characterization of perturbations in the brownseed mutants of tomato. Journal of Experimental Botany 55: 961-973. PDF
Dutt, M., G.A. Barthe, V. Orbovic M. Irey and J.W. Grosser (2013) Evaluation of transgenic citrus for disease resistance to HLB and Canker. Acta Horticulturae (submitted)
Dutt, M. and J.W. Grosser. 2011. Agrobacterium Mediated Transformation of Citrus Cell Suspension Cultures. Acta Horticulturae 892:119-124. PDF
Grosser, J.W., M. Dutt, A. Omar, V. Orbovic, G. Barthe. 2011. Progress towards the development of transgenic disease resistance in citrus. Acta Horticulturae 892: 101-107. PDF
Dutt, M., A.A. Omar, V. Orbovic, G. Barthe and J.W. Grosser. 2010. Progress towards incorporation of antimicrobial peptides for disease resistance in citrus. Proceedings of the 11th International Citrus Congress pp. 258-264. PDF
Dutt, M., G. Barthe and J.W. Grosser. 2010. Systemic acquired resistance (SAR) in citrus: Genetic transformation with SAR genes to combat Huanglongbing and Canker. Proceedings of the 11th International Citrus Congress pp. 289-294. PDF
Dutt, M., V. Orbovic, and J.W. Grosser. 2009. Cultivar dependent gene transfer into citrus using Agrobacterium. Proceedings of the Florida State Horticultural Society 122: 85 – 89. PDF
Dutt, M., A.A. Omar, V. Orbovic, G. Barthe, J. Gmitter, M. Vasconcellos, C. Dunning and J.W. Grosser. 2008. Combating Huanglongbing and Canker via Genetic Engineering of Citrus. Proceedings of the Florida State Horticultural Society 121:124-129. PDF
Geneve, R. L. and M. Dutt. 2008. A closer look at seed germination and dormancy. Combined Proceedings International Plant Propagator's Society 58: 561-565. PDF
Dutt, M., Z. T. Li, K. Kelley, S. A. Dhekney, M. Van Aman, J. Tattersall and D. J. Gray. 2007. Transgenic rootstock protein transmission in grapevines. Acta Horticulturae 738: 749-753. PDF
Li, Z. T., S. A. Dhekney, M. Dutt, M. Van Aman, J. Tattersall, K. Kelley and D. J. Gray. 2007. Isolation and characterization of the 2S albumin gene and promoter from grapevine. Acta Horticulturae 738: 759-765. PDF
Dhekney, S. A., Z. T. Li., M. Van Aman, M. Dutt, J. Tattersall, and D. J. Gray. 2007. Genetic transformation of embryogenic cultures and recovery of transgenic plants in Vitis vinifera, Vitis rotundifolia and Vitis hybrids. Acta Horticulturae 738: 743 - 748. PDF
Dutt, M., S. T. Kester and R. L. Geneve. 2004. Elevated levels of ethylene during germination reduces the time to radicle emergence in impatiens. Acta Horticulturae 631: 43 – 47. PDF
Dutt, M. and R. L. Geneve. 2002. Use of computer imaging to evaluate the initial stages of germination in woody tree seeds. Combined Proceedings International Plant Propagator's Society 52: 484-488. PDF
Dutt, M. and R. L. Geneve. 2002. Using sequential digital images captured with a flat bed scanner to evaluate woody plant seeds with different germination requirements. Proceedings of Southern Nursery Association Research Conference 47: 352-356. PDF
Dutt, M., Z.T. Li, S.A. Dhekney and D.J. Gray. 2012. Co-transformation of grapevine somatic embryos to produce transgenic plants free of marker genes In: J. Dunwell and A. Wetten (eds.).Transgenic Plants: Methods and Protocols. Springer New York pp. 201-213. PDF
Dhekney, S.A., Z.T. Li, M. Dutt and D.J. Gray. 2012. Initiation and transformation of grapevine embryogenic cultures. In: J. Dunwell and A. Wetten (eds.).Transgenic Plants: Methods and Protocols. Springer New York pp. 215-225. PDF
Geneve, R.L., M. Dutt, and A.B. Downie. 2006. Development of a sequential digital imaging system for evaluating seed germination. In: S. Navie, S. Adkins and S. Ashmore (eds.). Seeds: Biology, Development and Ecology. CAB International. London. pp. 315-323. PDF
Siriwitayawan, G., M. Dutt, S. T. Kester, A. B. Downie, and R. L. Geneve. 2003. Ageing in tomato reduces the capacity of seeds to produce ethylene, while priming increases ethylene evolution during germination. In: K. J. Bradford, D. Come, G. Nicolas and H. Pritchard (eds.) The Biology of Seeds: Recent Research Advances. CAB International, p.p. 441-446. PDF
- Society for In Vitro Biology
- American Society for Horticultural Science
- The Horticultural Society of India