Dr. Manjul Dutt
Contact Information
Citrus Research and Education Center
Lake Alfred, Florida
Horticulture
Phone: 863.956.1151 ext. 1472
Email: manjul@ufl.edu
Education
Ph.D. Horticulture, University of Florida.
Dissertation Title: Methods to genetically transform grapevines
M.S. Plant and Soil Science, University of Kentucky.
B.Sc. Agriculture, Orissa University of Agriculture and Technology, Bhubaneswar, India.
Positions
2007 onwards: Post-Doctoral Associate, Department of Horticultural Science, University of Florida, Citrus Research and Education Center (CREC), Lake Alfred, FL, USA.
2003 - 2006: Graduate Research Assistant, Department of Horticulture Sciences, University of Florida, Mid-Florida Research and Education center (MREC), Apopka, FL, USA.
2001-2003: Graduate Research Assistant, Department of Horticulture, University of Kentucky, Lexington, KY, USA.
Specialization
Plant tissue culture; Plant genetic engineering; Plant molecular biology and Seed physiology
Ongoing Research
- Design and construction of antibacterial peptide genes for resistance to citrus greening and canker.
- Genetic engineering of citrus for resistance to citrus greening and canker.
- Cloning and evaluation of phloem specific and inducible promoters in citrus.
Refereed Publications
Dutt, M., Z.T. Li, S.A. Dhekney and D.J. Gray (2008) A co-transformation system to produce transgenic grapevines free of marker genes. Plant Science (accepted).
Dutt, M. and R.L. Geneve (2008) Using sequential digital imagery to evaluate water movement in honeylocust seeds during development and imbibition. Seed Science and Technology (submitted).
Geneve, R.L. and M. Dutt (2008) Using sequential digital images to study seed germination and dormancy. Propagation of Ornamental Plants 8(1):13-16.
Dhekney, S.A., Z.T. Li, M. Dutt and D.J. Gray (2008) Agrobacterium-mediated transformation of embryogenic cultures and plant regeneration in Vitis rotundifolia Michx. (Muscadine grape). Plant Cell Reports (2008) 27(5):865-72.
Li, Z.T., S.A. Dhekney, M. Dutt, T.W. Zimmerman, and D.J. Gray (2008) An improved protocol for Agrobacterium-mediated transformation of grapevine. Plant Cell, Tissue and Organ Culture 93(3):311-321.
Dutt M., Z.T. Li, S.A. Dhekney and D.J. Gray (2007) Transgenic plants from shoot apical meristems of Vitis vinifera L. “Thompson Seedless” via Agrobacterium-mediated transformation. Plant Cell Reports 26(12):2101-2110.
Dutt, M., Z.T. Li, K. Kelley, S.A. Dhekney, M. Van Aman, J. Tattersall and D.J. Gray (2007) Transgenic rootstock protein transmission in grapevines. Acta Horticulturae 738:749-754.
Dutt, M. and R.L. Geneve (2007) Using sequential imagery to evaluate germination rate and seedling growth in seeds of different vigor. Journal of the American Society for Horticultural Sciences 132:423-428.
Li, Z.T., D.J. Gray, S.A. Dhekney, M. Dutt, M. Van Aman, J. Tattersall and K.T. Kelley (2007) Isolation and characterization of the 2S albumin gene and promoter from grapevine. Acta Horticulturae 738:759-765.
Dhekney, S.A., Z.T. Li., M. Van Aman, M. Dutt, J. Tattersall, and D.J. Gray (2007) Genetic transformation of embryogenic cultures and recovery of transgenic plants in Vitis vinifera, Vitis rotundifolia and Vitis hybrids. Acta Horticulturae 738:743-748.
Li, Z.T., S. Dhekney, M. Dutt, M. Van Aman, J. Tattersall, K.T. Kelley, and D.J. Gray (2006) Optimizing Agrobacterium-mediated transformation of grapevine. In Vitro Cellular & Developmental Biology - Plant 42(3):220-227.
Downie, A.B., L.M.A. Dirk, Q. Xu, J. Drake, M. Dutt, A. Levy, D.A. Butterfield, R.L. Geneve, J.W. Corum III, K.G. Lindstrom, and J.C. Snyder (2004) A physical, enzymatic, and genetic characterization of perturbations in the brownseed mutants of tomato. Journal of Experimental Botany 55:961-973.
Dutt, M., S.T. Kester and R.L. Geneve (2004) Elevated levels of ethylene during germination reduces the time to radicle emergence in impatiens. Acta Horticulturae 631:43-47.
Non-Referred Publications
Dutt, M., A.A. Omar, V. Orbovic, G. Barthe, J. Gmitter, M. Vasconcellos, C. Dunning and J.W. Grosser (2008) Combating Huanglongbing and Canker via Genetic Engineering of Citrus. Proceedings of the Florida State Horticultural Society (Submitted).
Dutt, M. and R.L. Geneve (2002) Use of computer imaging to evaluate the initial stages of germination in woody tree seeds. Combined Proceedings International Plant Propagator's Society 52:484-488.
Dutt, M. and R.L. Geneve (2002) Using sequential digital images captured with a flat bed scanner to evaluate woody plant seeds with different germination requirements. Proceedings of Southern Nursery Association Research Conference 47:352-356.
Selected Abstracts
Dutt,M., A.A. Omar, V. Orbovic, G. Barthe and J.W. Grosser (2008) Progress towards incorporation of antimicrobial peptides for disease resistance in citrus. (11th International Citrus Congress).
Dutt,M., G. Barthe and J.W. Grosser (2008) Systemic acquired resistance (SAR) in citrus: Genetic transformation with SAR genes to combat Huanglongbing and Canker. (11th International Citrus Congress).
Omar, A.A., M. Dutt, G. Barthe, V. Orbovic and J.W. Grosser (2008) Phloem specific transgene expression driven by AtSUC2 gene promoter in transgenic citrus plants to develop citrus greening resistance. In Vitro Cellular & Developmental Biology 44:S54
Gray, D. J., Z. T.Li, S. A. Dhekney, M. Dutt, D. Hopkins and T. W. Zimmerman (2007) Field testing of transgenic grapevine for bacterial and fungal disease resistance. HortScience 42(4):858.
Dutt, M., D. J. Gray, Z. T. Li, S. Dhekney and M. M. Van Aman (2006) Micropropagation cultures for genetic transformation of grapevine. HortScience 41(4):972.
Dutt, M., Z. T. Li, S. Dhekney and D. J. Gray (2006) Characterization of a composite promoter from genomic sequences of grapevine. HortScience 41(4):1053.
Dhekney, S.A., Z. T. Li, M. Dutt, M. Van Aman and D. J. Gray (2005) Genetic transformation and transgenic plant recovery from species of grape. In Vitro Cellular & Developmental Biology - Plant. 41:29.
Gray, D. J., Z. T. Li, D. L. Hopkins, M. Dutt, S. A. Dhekney, M. Van Aman, J. Tattersall and K. T. Kelley (2005) Transgenic Grapevines Resistant to Pierce’s Disease. HortScience 40(4):1104.
Dutt, M., Z. T. Li and D .J. Gray (2004) Development of an alternate genetic transformation system for Vitis. Florida Scientist 67. Supl. 1 :1.
Dutt, M., M. Van Aman and D. J. Gray (2004) Liquid culture for rapid in vitro propagation of Vitis. In Vitro Cellular & Developmental Biology - Plant. 40:56.
Dutt, M. and R. L. Geneve (2003) Germination rate and seedling growth rate measures different aspects of seed vigor in Impatiens. HortScience 38(5):781.
Book Chapters
Geneve, R.L., M. Dutt, and A.B. Downie (2006) Development of a sequential digital imaging system for evaluating seed germination. In: S. Navie, S. Adkins and S. Ashmore (eds.). Seeds: Biology, Development and Ecology. CAB International. London. pp. 315-323.
Siriwitayawan, G., M. Dutt, S.T. Kester, A.B. Downie, and R.L. Geneve (2003) Seed ageing in tomato reduces the capacity of seeds to produce ethylene, while priming increases ethylene evolution during germination. In: K. J. Bradford, D. Come, G. Nicolas and H. Pritchard (eds.) The Biology of Seeds: Recent Research Advances. CAB International. pp. 441-446.
Patent
D.J. Gray and M. Dutt. Genetic Transformation of Grapevines. US patent application 20070283455.
Professional Membership
- International Society for Horticultural Science
- American Society for Horticultural Science
- Florida State Horticultural Society
- The Horticultural Society of India
