Microscopy Lab

SEM Course Outline


    Week 1 - Lecture (Chapter 5)

  • Fixation including standard fixation and alternative techniques
  • Dehydration
  • Critical point drying and freeze drying
  • Mounting and storage of tissue
  • Sputter coating
  • Lab
  • Location of chemicals and supplies
  • Demonstrations: critical point dryer, sputter coater
  • Week 2 - Lecture (Chapters 1, 2)

  • Principles of SEM
  • Electron optics
  • Electromagnetic lenses
  • SEM Imaging Process
  • Vacuum System
  • Lab
  • Basic use of the SEM
  • Individual instruction
  • Week 3 - Lecture (Chapters 3, 4)

  • Principles of SEM
  • Electron beam dynamics – x-ray microanalysis, auger, etc.
  • Lab
  • Photographing electron micrographs
  • Use of apertures, working distance and condensor lens setting for increasing depth of field and resolution
  • Stigmation
  • Artifacts
  • Week 4 - Lecture (Chapter 6)

  • Basic Photography
  • Lab
  • Darkroom procedures and digital imaging
  • Week 5 - Lab (Chapter 7)

  • Preparation and copy of plates for publication
  • Preparation of digital media for presentation
  • Week 6 - Individual help on finishing projects