Transformation Lab

Citrus Transformation Phases

 

Sterilized seeds are allowed to germinate in darkness for 4-5 weeks

Sterilized seeds are allowed to germinate in darkness for 4-5 weeks

Stems of seedlings grown in vitro are cut into 1/2 inch explants, incubated with Agrobacteria and placed on shoot regeneration medium

Stems of seedlings grown in vitro are
cut into 1/2 inch explants, incubated with
Agrobacteria and placed on shoot
regeneration medium

Shoots appear in explants (4-5 weeks after treatment with bacteria)

Shoots appear in explants (4-5 weeks after treatment with bacteria)

Shoots are collected from explants and allowed to grow for a week

Shoots are collected from explants
and allowed to grow for a week

Tested for reporter genes

Tested for reporter genes

Shoots grafted on rootstocks in vitro

Shoots grafted on rootstocks in vitro

Two weeks old, soil adapted seedlings

Two weeks old, soil adapted seedlings

Four months seedlings

Four months seedlings

Gel with the products of PCR reaction proving that the gene of interest is present within the tissue of transgenic plants

Gel with the products of PCR reaction proving that the gene of interest is present within the tissue of transgenic plants