Publications - Faculty - Recently Submitted Faculty Publications 2008
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Reyes - Metabolomics
GC-MS analysis of headspace and liquid extracts for identification of potential biomarkers of citrus Huanglongbing in leaves of sweet orange
Juan Manuel Cevallos-Cevallos, Rosalía García-Torres, Edgardo Etxeberria, José I. Reyes-De-Corcuera.
Abstract. Citrus Huanglongbing (HLB) is considered the most destructive citrus disease worldwide. Rapid methods for early detection are needed to reduce HLB spread. GC-MS based methods for untargeted metabolite analysis of citrus leaves were developed and optimized. Sample extracts from healthy, zinc deficient, or HLB-infected sweet orange leaves were submitted to headspace solid phase micro-extraction (SPME) and derivatization treatments prior to GC-MS analysis. Principal components analysis achieved correct classification of all the derivatized liquid extracts but was not able to discriminate healthy from zinc deficient samples run with SPME. Analysis of variance revealed 6 possible biomarkers for HLB, of which 5 were identified as L-proline, β-elemene, (-)trans- caryophyllene, and α-humulene. Significant (P < 0.05) differences in oxo-butanedioic acid, arabitol, and neo-inositol were exclusively detected in zinc samples from plants with zinc deficiency. Levels of isocaryophyllen, α-selinene, β-selinene, and fructose were significantly (p < 0.05) different in healthy leaves only. Results suggest the potential of using identified HLB biomarkers for rapid detection of HLB.
