Skip to main content


UF/IFAS Citrus Research and Education Center

UF/IFAS Citrus Research and Education Center

Electropenetrography (EPG)

Program Agenda

The 2024 EPG Conference will be held for the second time at the University of Florida’s Citrus Research and Education Center in Lake Alfred, Florida, USA from January 29 - February 2, 2024.

Monday, January 29, 2024

8:00 am - 9:00 am  Registration/Help Desk
9:00 am - 12:00 pm EPG Pre-Conference Hands-on Wiring and Recording Assistance
(Preregistration required)
12:00 pm - 1:00 pm Lunch
1:00 pm - 5:00 pm EPG Pre-Conference Hands-on Wiring and Recording Assistance
(Preregistration required)
 

Tuesday, January 30, 2024

8:00 am - 9:00 am Registration/Help Desk
9:00 am - 9:05 am Overview of the Week
Lauren Diepenbrock, UF/IFAS CREC
9:05 am - 9:25 am Welcome to CREC and EPG Conference
Michael Rogers, UF/IFAS CREC
9:25 am - 10:00 am Overview of History and Principles of EPG
Elaine Backus, USDA Agricultural Research Service, Parlier, CA
10:00 am - 10:20 am Break
10:20 am - 11:20 am Advancements in AC-DC electropenetrography accelerate application of EPG to all-new arthropod pests and research questions
Elaine Backus, USDA Agricultural Research Service, Parlier, CA
View Abstract
11:20 am - 11:40 am Can entomopathogenic fungi alter the feeding behavior of the neotropical brown-stink bug?
Andre Cirilo de Sousa Almeida, Instituto Federal Goiano, Brazil
View Abstract
11:40 am - 12:00 pm Using EPG to determine the duration of feeding associated with scarring damage in faba bean seed
Teresa Aguiler-Cordero, University of Saskatchewan, Canada
View Abstract
12:00 pm – 1:30 pm Lunch
1:30 pm - 1:50 pm Introduction to Blood Feeders
Kathryn Reif, Auburn University
1:50 pm - 2:10 pm Pushing past the limits: Application of electropenetrography to unmask the temporal details of tick feeding behaviors
Kathryn Reif, Auburn University
View Abstract
2:10 pm - 2:30 pm A waveform library for Culex tarsalis mosquitoes probing on human hands
Anastasia Cooper, Kansas State University
View Abstract
2:30 pm - 3:00 pm Break
3:00 pm - 3:20 pm Waveforms from Culicoides sonorensis biting midges probing on human hands
Anastasia Cooper, Kansas State University
View Abstract
3:20 pm - 3:40 pm Using EPG to examine dengue virus transmission in a mouse model
Samuel Jameson, Tulane University
View Abstract
3:40 pm - 4:00 pm Tackling food insecurity in drought-hit areas of District
Tharparkar, Sindh, Pakistan: implications for agricultural extension
Nadeem Abbas, Affiliation unknown
View Abstract
4:00 pm - 5:00 pm Discussion of challenges in adopting EPG to novel arthropods and applications
Elaine Backus, USDA Agricultural Research Service, Parlier, CA, and Kathryn Reif, Auburn University
   

Wednesday, January 31, 2024

8:00 am - 9:00 am Registration/Help Desk
9:00 am - 10:00 am Whole tissue histology and microscopy techniques for rapid electropenetrography feeding site correlations in plant or animal tissues
Holly Shugart, Pennsylvania State University
View Abstract
10:00 am - 10:20 am Characterization and correlation of the probing behaviors of Macrosteles quadrilineatus with EPG waveforms
Berenice Romero, University of Saskatchewan
View Abstract
10:20 am - 10:40 am Histology Discussion
Holly Shugart, Pennsylvania State University
10:45 am - 11:15 am

UF/IFAS CREC Microscopy Core Tour
Holly Shugart and Daniel Stanton, UF/IFAS CREC

11:20 am - 12:00 pm Poster Session, Open Discussion
12:00 pm - 1:00 pm Lunch
1:00 pm - 5:00 pm Circle B Reserve
   

Thursday, February 1, 2024

8:00 am - 9:00 am Registration/Help Desk
9:00 am - 10:00 am INFEST: Insect Feeding Behavior Statistics
Anderson da Silva, Instituto Federal Goiano, Brazil
View Abstract
10:00 am - 10:20 am Planning sample size with reference to EPG experiments
Tim Ebert, UF/IFAS CREC
View Abstract
10:20 am - 10:50 am Break
10:50 am - 11:10 am Characterization of the feeding behavior of the Asian citrus psyllid, Diaphorina citri (Hemiptera: Liviidae)
on finger limes and finger lime hybrids using electropenetrography (EPG)
Emilie Demard, UF/IFAS CREC
View Abstract
11:10 am - 11:30 am The probing behavior and vector potential of Melanaphis sacchari in transmitting Sorghum mosaic virus
Jeff Davis, Louisiana State University Agricultural Center
View Abstract
11:30 am - 11:50 am Electropenetrography proves a plant bacterial manipulation of its Insect vector's energy metabolism
Nabil Killiny, UF/IFAS CREC
View Abstract
11:50 pm - 1:20 pm Lunch
1:20 pm - 2:20 pm Three-decade application and innovation of electrical penetration graph (EPG) technique in China
Fengming Yan, Henan Agricultural University, China
View Abstract
2:20 pm - 2:40 pm

Beet leafhopper (Circulifer tenellus; Hemiptera: Cicadellidae) probing behavior relative to Wolbachia infection

Gina Angelella, USDA Agricultural Research Service, Wapato, WA
View Abstract

2:40 pm - 3:10 pm Break
3:10 pm - 3:30 pm Exploring the interplay of EPG, PSbMV, and aphids as vectors in pea plants
Simin Sabaghian, University of Saskatchewan, Canada
View Abstract
3:30 pm - 3:50 pm Use of electropenetrography to evaluate pea aphid feeding behavior on resistant lentil lines in Saskatchewan
Rodensky Teramene, University of Saskatchewan, Canada
View Abstract
3:50 pm - 4:50 pm Discussion of challenges in statistically analyzing EPG data
Elaine Backus, USDA Agricultural Research Service; Anderson da Silva, Instituto Federal Goiano; Tim Ebert, UF/IFAS CREC
   

Friday, February 2, 2024

8:00 am - 9:00 am Registration/Help Desk
9:00 am - 12:00 pm

EPG Post-Conference Statistical Analysis Assistance (Preregistration required)
Measurement of AC-DC EPG waveforms and data formatting
Elaine Backus, USDA Agricultural Research Service, Parlier, CA


Using R INFEST to analyze non-sequential variables 
Anderson da Silva, Instituto Federal Goiano, Brazil


Using SAS to analyze sequential variables
Tim Ebert, UF/IFAS CREC

12:00 pm - 1:00 pm Lunch
1:00 pm - 5:00 pm EPG Post-Conference Statistical Analysis Assistance (Preregistration required)
Statistics Assistance one-on-one: Bring your own data and your own computer with R and/or SAS installed!